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Ether Sedimentation Ethyl

Formal Ether Sedimentation technique for the concentration ...

2012-08-03  Principle of Formal ether (Formalin-Ethyl Aceatate) sedimentation technique Sedimentation techniques use solutions of lower specific gravity than the parasitic organisms, thus concentrating the latter in the sediment. It takes advantage of the high specific gravity of protozoan cysts and helminth eggs compared to water.

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Comparison of clinical results for the use of ethyl ...

Abstract One hundred fecal specimens preserved in polyvinyl alcohol fixative were examined by the Formalin-ether sedimentation technique with ethyl acetate substituted for diethyl ether. Technical performance of the procedures, appearance and amount of sediment obtained, and organism morphology were comparable.

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Formal Ether Sedimentation stool concentration technique ...

2018-04-12  The formol-ether method is a widely used sedimentation technique for stool examination. Principle of Formal ether sedimentation technique The Sedimentation techniques makes uses solutions of lower specific gravity as compare to the parasites, thus concentrating the latter in

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Formal Ether Sedimenation Technique ~ University Notes

Concentration technique increase the sensitivity Formal ether sedimentation technique is a commonly used concentration technique. Fig 1: Formal Ether Sedimentation Technique : Principle of Formal ether (Formalin-Ethyl Aceatate) sedimentation technique. Formalin fixes the eggs, larvae, oocysts, and spores, so that they are no longer infectious, as well as preserves their morphology. Fecal ...

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Ethyl acetate as a substitute for diethyl ether in the ...

1979-12-01  Ethyl acetate appears to be a satisfactory subsitute solvent for diethyl ether in the Formalin-ether sedimentation technique. In comparative studies, concentration of organisms with ethyl acetate was equal to or greater than that with diethyl ether.

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[PDF] Comparison of formalin-ethyl ether sedimentation ...

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered.

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Comparison of Formalin-Ethyl Ether Sedimentation, Formalin ...

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitol- ogy specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were en-

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CDC - DPDx - Diagnostic Procedures - Stool Specimens

2016-05-03  Add 10 ml of 10% formalin to the sediment and mix thoroughly with wooden applicator sticks. Add 4 ml of ethyl acetate, stopper the tube, and shake vigorously in an inverted position for 30 seconds. Carefully remove the stopper. Centrifuge at 500 × g for 10 minutes.

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Comparison of Formalin-Ethyl Ether Sedimentation, Formalin ...

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitol-ogy specimens. Fifty positive specimens were identified, and a variety of parasites,

More

Ethyl acetate as a substitute for diethyl ether in the ...

Ethyl acetate appears to be a satisfactory subsitute solvent for diethyl ether in the Formalin-ether sedimentation technique. In comparative studies, concentration of organisms with ethyl acetate was equal to or greater than that with diethyl ether. No distortion or alteration of morphology was observed with eigher solvent, and preparations were comparable in appearance and ease of examination ...

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[PDF] Comparison of formalin-ethyl ether sedimentation ...

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered. The Formalin-ether and Formalin-ethyl acetate sedimentation ...

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Formal Ether Sedimenation Technique ~ University Notes

Concentration technique increase the sensitivity Formal ether sedimentation technique is a commonly used concentration technique. Fig 1: Formal Ether Sedimentation Technique : Principle of Formal ether (Formalin-Ethyl Aceatate) sedimentation technique. Formalin fixes the eggs, larvae, oocysts, and spores, so that they are no longer infectious, as well as preserves their morphology. Fecal ...

More

CDC - DPDx - Diagnostic Procedures - Stool Specimens

The sedimentation technique used at CDC is the formalin-ethyl acetate technique, a diphasic sedimentation technique that avoids the problems of flammability of ether, and which can be used with specimens preserved in formalin, MIF or SAF. Formalin-Ethyl Acetate Sedimentation Concentration. Mix the specimen well. Strain 5ml of the fecal suspension (more or less depending on its consistency ...

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Concentration Techniques- sedimentation

Modified Formal- Ether Sedimentation. Formalin- Ether or Formalin- Ethyl acetate method is the recommended concentration procedures. Most types of worm eggs (round worms, tapeworms, schistosomes, and other fluke eggs), larvae, and protozoan cysts may be recovered by this method. Advantages: Speed: one sample can be processed in 5 minutes. Broad spectrum: it will recover most

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Concentration Techniques : Introduction, Principle, Types ...

Formalin- Ether or Formalin- Ethyl acetate method is the recommended concentration technique. Most types of worm eggs (roundworms, tapeworms, schistosomes, and other fluke eggs), larvae, and protozoan cysts may be recovered by this method. Principle of Formal-Ether Concentration Technique. This type of concentration technique clears from its name using formalin and ether. Sedimentation ...

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An ether sedimentation technique for routine stool ... - OCLC

An ether sedimentation technique for routine stool examinations. An ether sedimentation technique for routine stool examinations. Search this record. Item Description. Title : An ether sedimentation technique for routine stool examinations: Author(s) Ritchie, Lawrence S. Journal: Bulletin of the U.S. Army Medical Department, Vol. 8, no. 4 (Apr. 1948), p. 326, (OCoLC) 01606253: Subject ...

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Formol-ether concentration method in the diagnosis of ...

Comparison of formalinethyl ether Sedimentation, formalin ethyl acetate sedimentation and zinc sulfate flotation technique for detection of intestinal parasite. J Clin Mic 1981;13:882-4. 9. Dos-Santos JG, Watson B. Direct wet mounts versus concentration for routine parasitologic examination are both necessary. Am J Clin Pathology 1988;89:389-91. 10. Elliott EE. Schistosomiasis pathophysiology ...

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Faecal Parasitology: Concentration Methodology Needs to be ...

2016-04-13  Two mL of ether or ethyl acetate was added (formalin/Triton-X 100 mixture was added to the mixture if ethyl acetate was used as it helps to emulsify the faecal matter). Parasep was assembled and sealed by screwing the filter thimble and sedimentation cone onto the mixing chamber. The mixture was vortexed for 15 seconds and the system inverted to allow the mixture to be filtered through the ...

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CDC - DPDx - Diagnostic Procedures - Stool Specimens

The sedimentation technique used at CDC is the formalin-ethyl acetate technique, a diphasic sedimentation technique that avoids the problems of flammability of ether, and which can be used with specimens preserved in formalin, MIF or SAF. Formalin-Ethyl Acetate Sedimentation

More

Formal Ether Sedimenation Technique ~ University Notes

Concentration technique increase the sensitivity Formal ether sedimentation technique is a commonly used concentration technique. Fig 1: Formal Ether Sedimentation Technique : Principle of Formal ether (Formalin-Ethyl Aceatate) sedimentation

More

Concentration Techniques- sedimentation

Modified Formal- Ether Sedimentation. Formalin- Ether or Formalin- Ethyl acetate method is the recommended concentration procedures. Most types of worm eggs (round worms, tapeworms, schistosomes, and other fluke eggs), larvae, and protozoan cysts may be recovered by this method. Advantages: Speed: one sample can be processed in 5 minutes. Broad spectrum: it will recover most

More

Ethyl acetate as a substitute for diethyl ether in the ...

1979-12-01  Ethyl acetate appears to be a satisfactory subsitute solvent for diethyl ether in the Formalin-ether sedimentation technique. In comparative studies, concentration of organisms with ethyl acetate was equal to or greater than that with diethyl ether. No distortion or alteration of morphology was observed with eigher solvent, and preparations were comparable in appearance and

More

Comparison of formalin-ethyl ether sedimentation, formalin ...

"Formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered. The formalin-ether and formalin-ethyl acetate sedimentation...

More

An ether sedimentation technique for routine stool ... - OCLC

An ether sedimentation technique for routine stool examinations. An ether sedimentation technique for routine stool examinations. Search this record. Item Description. Title : An ether sedimentation technique for routine stool examinations: Author(s) Ritchie, Lawrence S. Journal: Bulletin of the U.S. Army Medical Department, Vol. 8, no. 4 (Apr. 1948), p. 326, (OCoLC) 01606253: Subject ...

More

Adaptation of Ritchie's Method for Parasites Diagnosing ...

L. S. Garcia and R. Shimizu, “Comparison of clinical results for the use of ethyl acetate and diethyl ether in the formalin-ether sedimentation technique performed on polyvinyl alcohol-preserved specimens,” Journal of Clinical Microbiology, vol. 13, no. 4, pp. 709–713, 1981. View at: Google Scholar

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Formol-ether concentration method in the diagnosis of ...

Comparison of formalinethyl ether Sedimentation, formalin ethyl acetate sedimentation and zinc sulfate flotation technique for detection of intestinal parasite. J Clin Mic 1981;13:882-4. 9. Dos-Santos JG, Watson B. Direct wet mounts versus concentration for routine parasitologic examination are both necessary. Am J Clin Pathology 1988;89:389-91. 10. Elliott EE. Schistosomiasis pathophysiology ...

More

Comparison of 2 centrifugation procedures in the formalin ...

1996-06-01  An ether sedimentation technique for routine stool examinations. Bulletin of the U.S. Army Medicine Department 8: 326. Weber R., Bryan R. T., Bishop H. S., Wahlquist S. P., Sullivan J. J. Juranek D. D. 1991. Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity of current diagnostic methods. Journal of Clinical Microbiology 29: 1323

More

Faecal Parasitology: Concentration Methodology Needs to be ...

2016-04-13  Two mL of ether or ethyl acetate was added (formalin/Triton-X 100 mixture was added to the mixture if ethyl acetate was used as it helps to emulsify the faecal matter). Parasep was assembled and sealed by screwing the filter thimble and sedimentation cone onto the mixing chamber. The mixture was vortexed for 15 seconds and the system inverted to allow the mixture to be filtered through the ...

More

Comparison of formalin-ethyl ether sedimentation, formalin ...

"Formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered. The formalin-ether and formalin-ethyl acetate sedimentation...

More

Re-evaluation of the formalin-ether sedimentation method ...

The formalin-ether sedimentation (FES) method is considered as reliable method of fecal examination for the detection of parasites. In this study, we re-evaluated several aspects of FES such as (i) pretreatment of feces; (ii) fi ltration of fecal suspensions; (iii) test-tube material and (iv) substitution of ether by other organic solvents as to see an improvement in parasite egg recovery ...

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An ether sedimentation technique for routine stool ... - OCLC

An ether sedimentation technique for routine stool examinations. An ether sedimentation technique for routine stool examinations. Search this record. Item Description. Title : An ether sedimentation technique for routine stool examinations: Author(s) Ritchie, Lawrence S. Journal: Bulletin of the U.S. Army Medical Department, Vol. 8, no. 4 (Apr. 1948), p. 326, (OCoLC) 01606253: Subject ...

More

Comparison of clinical results for the use of ethyl ...

"One hundred faecal specimens preserved in polyvinyl alcohol fixative were examined by the formalin-ether sedimentation technique with ethyl acetate substituted for diethyl ether. Technical performance of the procedures, appearance and amount of sediment obtained, and organism morphology were comparable. Also, ethyl acetate is less flammable and, therefore, less dangerous to use than diethyl...

More

Comparison of 2 centrifugation procedures in the formalin ...

1996-06-01  An ether sedimentation technique for routine stool examinations. Bulletin of the U.S. Army Medicine Department 8: 326. Weber R., Bryan R. T., Bishop H. S., Wahlquist S. P., Sullivan J. J. Juranek D. D. 1991. Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity of current diagnostic methods. Journal of Clinical Microbiology 29: 1323

More

Adaptation of Ritchie's Method for Parasites Diagnosing ...

L. S. Garcia and R. Shimizu, “Comparison of clinical results for the use of ethyl acetate and diethyl ether in the formalin-ether sedimentation technique performed on polyvinyl alcohol-preserved specimens,” Journal of Clinical Microbiology, vol. 13, no. 4, pp. 709–713, 1981. View at: Google Scholar

More

Formol-ether concentration method in the diagnosis of ...

Comparison of formalinethyl ether Sedimentation, formalin ethyl acetate sedimentation and zinc sulfate flotation technique for detection of intestinal parasite. J Clin Mic 1981;13:882-4. 9. Dos-Santos JG, Watson B. Direct wet mounts versus concentration for routine parasitologic examination are both necessary. Am J Clin Pathology 1988;89:389-91. 10. Elliott EE. Schistosomiasis pathophysiology ...

More

FECAL CONCENTRATION (MICRO-SED, 15 ML) - Med-Chem

layers: Top: ethyl acetate or ethyl ether; Second: debris plug; Third: saline*; and Fourth: fecal sediment. N. Ring the debris layer with an applicator stick to loosen the debris. O. Invert the tube to pour off the supernatant fluid and debris layer. P. While the tube is still inverted, wipe the sides of the tube with one or two cotton -tipped applicators to remove ethyl acetate or debris left ...

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TECHNICAL REPORT EVALUATION OF THE FORMALIN-TWEEN ...

The formalin-Tween sedimentation method was compared with the formalin-ether sedimentation for parasitic detection. Of a total 297 fecal specimens examined, 72.1% were positive. The formalin-tween technique was effective for ascertaining helminths, particularly Ascaris lumbricoides, Trichuris trichiura and hookworm eggs; however it has less capability for protozoa detection. This method is ...

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